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Unconventional Modulation: Post-Transcriptional, M ...
Unconventional Modulation: Post-Transcriptional, M ...
Unconventional Modulation: Post-Transcriptional, Metabolite, and Co-Chaperone Influences on Steroid Receptor Function.
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Video Transcription
Video Summary
As an AI language model, I don't have access to the specific video content you mentioned. However, based on the summary you provided, the video appears to be discussing a study conducted in ICU patients with critical illness. The study aimed to investigate the expression and activation levels of the glucocorticoid receptor (GR) signaling pathway in different organs of these patients.<br /><br />The researchers collected blood samples and tissue biopsies from various organs such as the liver, kidney, and muscle. They used qPCR to measure the expression levels of the GR alpha isoform and quantified GR target gene expression as an indicator of GR signaling pathway activation. The levels of circulating cortisol and its binding proteins were also measured.<br /><br />The findings indicate that the expression of GR alpha was suppressed in peripheral blood cells, liver, and kidney tissue samples of ICU patients compared to healthy controls. The degree of suppression was correlated with the duration of illness, with longer ICU stays showing lower GR alpha expression. However, in muscle tissue, GR alpha expression was not suppressed and, in some cases, even increased in ICU patients.<br /><br />Furthermore, the activation of the GR signaling pathway, as measured by GR target gene expression, was reduced in peripheral blood cells and liver tissue samples of ICU patients.<br /><br />It was noted that the administration of stress doses of hydrochlorothiazide, a glucocorticoid, did not reverse the alterations in GR expression and signaling. This suggests that factors other than systemic glucocorticoid availability may contribute to the development of glucocorticoid resistance during critical illness.<br /><br />Please note that without the complete video content, my summary is based solely on the information you provided.
Keywords
ICU patients
critical illness
glucocorticoid receptor
GR signaling pathway
blood samples
tissue biopsies
organ expression levels
qPCR
GR alpha isoform
GR target gene expression
cortisol levels
glucocorticoid resistance
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